聚（1-甲基肌苷酸）的拉曼光谱研究。
Raman spectral studies of poly(1-methylinosinic acid).
作者信息
Scovell W M, Amarnath V, Broom A D
出版信息
Nucleic Acids Res. 1979 Mar;6(3):1049-61. doi: 10.1093/nar/6.3.1049.
The synthesis and characterization of poly(1-methylinosinic acid) are described. Laser Raman spectra of poly (mII) were obtained as a function of temperature in D2O solution. Thermal melting profiles derived from the intensity variations of the 712, 795, 814, 986, 1333, 1509, 1550 and 1680 cm-1 bands indicate a cooperative melting temperature of 9 +/- 1 degree C. The low temperature form of poly(mII) exhibits a carbonyl frequency at 1710 cm-I which is decreased to 1680 cm-I upon melting. The Raman hypochromism in the bands reported are equal to or much larger than any reported for other nucleic acids. The data are consistent with the low temperature form of poly(mII) being an ordered single stranded unit with a high degree of basestacking. The melting profiles obtained from the uv and cd spectra are consistent with and support the Raman data. This single stranded RNA exhibits an uncharacteristic behavior in that it melts cooperatively.
本文描述了聚（1-甲基肌苷酸）的合成与表征。在重水（D2O）溶液中，获得了聚（mII）的激光拉曼光谱随温度的变化情况。由712、795、814、986、1333、1509、1550和1680 cm-1波段强度变化得出的热熔解曲线表明，协同熔解温度为9±1℃。聚（mII）的低温形式在1710 cm-1处呈现羰基频率，熔解时该频率降至1680 cm-1。所报道波段的拉曼减色效应等于或远大于其他核酸报道的任何值。数据表明聚（mII）的低温形式是具有高度碱基堆积的有序单链单元。由紫外光谱和圆二色光谱获得的熔解曲线与拉曼数据一致并支持该数据。这种单链RNA表现出一种非典型行为，即它协同熔解。
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