Zhurkin V B, Lysov Y P, Ivanov V I
Nucleic Acids Res. 1979 Mar;6(3):1081-96. doi: 10.1093/nar/6.3.1081.
Potential energy calculations of the DNA duplex dimeric subunit show that the double helix may be bent in the direction of minor and major grooves much more easily than in other directions. It is found that the total winding angle of DNA decreases upon such bending. A new model for DNA folding in the nucleosome is proposed on the basis of these findings according to which the DNA molecule is kinked each fifth base pair to the side of the minor and major grooves alternatively. The model explains the known contradiction between a C-like circular dichroism for the nucleosomal DNA and the nuclease digestion data, which testify to the B-form of DNA.
对DNA双链二聚体亚基的势能计算表明，双螺旋向小沟和大沟方向弯曲可能比向其他方向弯曲更容易。研究发现，DNA的总缠绕角在这种弯曲时会减小。基于这些发现，提出了一种核小体中DNA折叠的新模型，根据该模型，DNA分子每隔五个碱基对交替向小沟和大沟一侧弯折。该模型解释了核小体DNA的C类圆二色性与核酸酶消化数据之间已知的矛盾，核酸酶消化数据证明DNA为B型。
