噬菌体λ中Rec介导的重组热点活性。II. 一个导致热点活性的突变。
Rec-mediated recombinational hot spot activity in bacteriophage lambda. II. A mutation which causes hot spot activity.
作者信息
Lam S T, Stahl M M, McMilin K D, Stahl F W
出版信息
Genetics. 1974 Jul;77(3):425-33. doi: 10.1093/genetics/77.3.425.
Abstract
Crosses have been performed which identify phage mutants (chi) which cause recombinational hot spot activity in lambda. The hot spot activity is found in crosses of red(-) gam(-) chi(-) strains in rec(+) hosts; in the crosses reported here, both the chi(-) mutations and the hot spot are located near the right end of the chromosome. The hot spot occurs in standard crosses as well as under conditions which block DNA synthesis, and is dependent on a functional host recB gene.-The chi mutation is shown to be dominant, but the tests do not show whether chi is a gene or a site.
摘要
已经进行了杂交实验，鉴定出导致λ噬菌体中重组热点活性的噬菌体突变体（chi）。在rec（+）宿主中红色（-）gam（-）chi（-）菌株的杂交中发现了热点活性；在此处报道的杂交中，chi（-）突变和热点都位于染色体右端附近。热点在标准杂交以及阻断DNA合成的条件下都会出现，并且依赖于功能性宿主recB基因。chi突变显示为显性，但这些测试并未表明chi是一个基因还是一个位点。
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本文引用的文献
1
Bacteriophage lambda; abortive infection of bacteria lysogenic for phage P2.噬菌体λ；对噬菌体P2呈溶原性的细菌的流产感染。
Proc Natl Acad Sci U S A. 1970 Jul;66(3):587-94. doi: 10.1073/pnas.66.3.587.
2
Characterization of two bacterial mutants with temperature-sensitive synthesis of DNA.两个具有温度敏感型DNA合成特性的细菌突变体的表征
Genetics. 1968 Sep;60(1):1-17. doi: 10.1093/genetics/60.1.1.
3
Requirement for maturation of Escherichia coli bacteriophage lambda.大肠杆菌噬菌体λ成熟的要求
J Mol Biol. 1974 Mar 15;83(4):503-9. doi: 10.1016/0022-2836(74)90510-5.
4
On maturation of the bacteriophage lambda chromosome.关于噬菌体λ染色体的成熟。
Mol Gen Genet. 1973 Dec 31;127(4):285-95. doi: 10.1007/BF00267099.
5
Replication of bacteriophage lambda DNA dependent on the function of host and viral genes. I. Interaction of red, gam and rec.噬菌体λDNA的复制依赖于宿主和病毒基因的功能。I. red、gam和rec的相互作用
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6
A role for recombination in the production of "free-loader" lambda bacteriophage particles.重组在“搭便车”λ噬菌体颗粒产生中的作用。
J Mol Biol. 1972 Jul 14;68(1):57-67. doi: 10.1016/0022-2836(72)90262-8.
7
Maturation and recombination of bacteriophage lambda DNA molecules in the absence of DNA duplication.噬菌体λ DNA分子在无DNA复制情况下的成熟与重组。
J Mol Biol. 1972 Jul 14;68(1):49-55. doi: 10.1016/0022-2836(72)90261-6.
8
An enhancing role for DNA synthesis in formation of bacteriophage lambda recombinants.DNA合成在噬菌体λ重组体形成中的增强作用。
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Proc Natl Acad Sci U S A. 1973 Aug;70(8):2215-9. doi: 10.1073/pnas.70.8.2215.
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