Reynolds M C, Lindell K F, David T J, Favero M S, Bond W W
Appl Microbiol. 1974 Sep;28(3):406-10. doi: 10.1128/am.28.3.406-410.1974.
Samples of soil collected from the Kennedy Space Center near the spacecraft assembly facilities were found to contain microorganisms very resistant to conventional sterilzation techniques. The inactivation kinetics of the naturally occurring spores in soil were investigated by using dry heat and ionizing radiation, first separately and then simultaneously. Dry-heat inactivation kinetics of spores was determined at 105 and 125 C; radiation inactivation kinetics was determined for dose rates of 660 and 76 krads/h at 25 C. Simultaneous combinations of heat and radiation were then investigated at 105, 110, 115, 120, and 125 C, with a dose rate of 76 krads/h. Combined treatment was found to be highly synergistic, requiring greatly reduced radiation doses to accomplish sterilization of the population.
从航天器组装设施附近的肯尼迪航天中心采集的土壤样本被发现含有对传统灭菌技术具有很强抗性的微生物。通过分别使用干热和电离辐射，然后同时使用这两种方法，研究了土壤中天然存在的孢子的失活动力学。在105℃和125℃下测定了孢子的干热失活动力学；在25℃下，针对660和76千拉德/小时的剂量率测定了辐射失活动力学。然后在105℃、110℃、115℃、120℃和125℃下，以76千拉德/小时的剂量率研究了热和辐射的同时组合。发现联合处理具有高度协同作用，完成群体灭菌所需的辐射剂量大大降低。
