一种通过免疫吸附技术分离单个多核糖体和纯mRNA的通用方法。 - Suppr

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超能文献

A general method for isolation of individual poly-ribosomes and pure mRNAs by immunosorption technique.

作者信息

Sidorova E V, Trudolyubova M G, Lerman M I

出版信息

Mol Biol Rep. 1974 Sep;1(7):401-8. doi: 10.1007/BF00385673.

DOI:10.1007/BF00385673

PMID:4422337

Abstract

摘要

相似文献

A general method for isolation of individual poly-ribosomes and pure mRNAs by immunosorption technique.一种通过免疫吸附技术分离单个多核糖体和纯mRNA的通用方法。

Mol Biol Rep. 1974 Sep;1(7):401-8. doi: 10.1007/BF00385673.

Immunoadsorption of ovalbumin synthesizing polysosmes and partial purification of ovalbumin messenger RNA.卵清蛋白合成多核糖体的免疫吸附及卵清蛋白信使核糖核酸的部分纯化

Methods Enzymol. 1974;30:631-48. doi: 10.1016/0076-6879(74)30061-4.

The separation and identification of alpha- and beta-globin messenger ribonucleic acids.α和β珠蛋白信使核糖核酸的分离与鉴定。

J Biol Chem. 1974 Aug 25;249(16):5290-5.

[Isolation of presumed albumin mRNA from rat liver].[从大鼠肝脏中分离假定的白蛋白信使核糖核酸]

Dokl Akad Nauk SSSR. 1975 Jun 1;222(4):987-90.

Isolation of messenger RNA from polysomes by chromatography on oligo(dT)-cellulose.通过在寡聚（dT）-纤维素上进行层析从多核糖体中分离信使核糖核酸。

Anal Biochem. 1975 May 26;66(1):18-28. doi: 10.1016/0003-2697(75)90720-4.

Isolation of messenger-like ribonucleoproteins.信使样核糖核蛋白的分离

Biochemistry. 1975 May 6;14(9):1815-21. doi: 10.1021/bi00680a002.

Distribution of histone messenger RNA among free and membrane-associated polyribosomes of a mouse myeloma cell line.组蛋白信使核糖核酸在小鼠骨髓瘤细胞系的游离及膜结合多核糖体中的分布

J Mol Biol. 1973 Sep 25;79(3):577-86. doi: 10.1016/0022-2836(73)90407-5.

Cell-free synthesis of myosin.肌球蛋白的无细胞合成

Methods Enzymol. 1974;30:669-74. doi: 10.1016/0076-6879(74)30064-x.

Protein synthesis with messenger ribonucleic acid fractions from membrane-bound and free liver polysomes. Translation characteristics of liver polysomal ribonucleic acids and evidence for albumin production in a messenger-dependent reticulocyte cell-free system.利用来自膜结合型和游离型肝脏多核糖体的信使核糖核酸片段进行蛋白质合成。肝脏多核糖体核糖核酸的翻译特性以及在信使依赖的无细胞网织红细胞系统中白蛋白产生的证据。

J Biol Chem. 1974 Jan 10;249(1):81-8.

Translation of immunoglobulin mRNAs in a wheat germ cell-free system.小麦胚无细胞体系中免疫球蛋白mRNA的翻译

Mol Biol Rep. 1974 Mar;1(6):355-63. doi: 10.1007/BF00309570.

引用本文的文献

Purification of low-abundance messenger RNAs from rat liver by polysome immunoadsorption.通过多核糖体免疫吸附法从大鼠肝脏中纯化低丰度信使核糖核酸

Proc Natl Acad Sci U S A. 1982 Jul;79(13):4015-9. doi: 10.1073/pnas.79.13.4015.

Three mRNA populations differing in turnover and processing in mouse liver.小鼠肝脏中在周转和加工方面存在差异的三种mRNA群体。

Mol Biol Rep. 1975 Dec;2(4):269-75. doi: 10.1007/BF00357013.

本文引用的文献

Incorporation of radioactive amino acids into serum albumin by isolated rat-liver ribosomes.用分离的大鼠肝脏核糖体将放射性氨基酸掺入血清白蛋白中。

Biochem J. 1962 Apr;83(1):69-74. doi: 10.1042/bj0830069.

USE OF ANTIBODIES ON AN INSOLUBLE SUPPORT FOR SPECIFIC DETECTION OF RADIOACTIVE ANTIGENS.抗体在不溶性载体上用于放射性抗原特异性检测的应用。

Nature. 1964 Aug 8;203:648-9. doi: 10.1038/203648a0.

Studies on free and membrane-bound ribosomes in rat liver. I. Distribution as related to total cellular RNA.大鼠肝脏中游离核糖体和附着核糖体的研究。I. 与细胞总RNA相关的分布情况

J Mol Biol. 1967 Jun 14;26(2):279-92. doi: 10.1016/0022-2836(67)90297-5.

Functional differences in protein synthesis between free and bound polysomes of rat liver.大鼠肝脏游离多核糖体与结合多核糖体之间蛋白质合成的功能差异。

Biochim Biophys Acta. 1970 Sep 17;217(1):148-58. doi: 10.1016/0005-2787(70)90131-0.

Dissociation of mammalian polyribosomes into subunits by puromycin.嘌呤霉素使哺乳动物多核糖体解离为亚基。

Proc Natl Acad Sci U S A. 1971 Feb;68(2):390-4. doi: 10.1073/pnas.68.2.390.

Precipitation of polyribosomes with pepsin digested antibodies.用胃蛋白酶消化的抗体沉淀多核糖体。

Biochem Biophys Res Commun. 1971 Oct 1;45(1):240-5. doi: 10.1016/0006-291x(71)90075-1.

Purification and properties of ovalbumin messenger RNA.卵清蛋白信使核糖核酸的纯化及性质

Eur J Biochem. 1974 Apr 16;43(3):549-60. doi: 10.1111/j.1432-1033.1974.tb03442.x.

Identification and isolation of ovalbumin-synthesizing polysomes. 3. Precipitation of ovalbumin polysomes from a heterologous cell-free protein-synthesizing system.卵清蛋白合成多核糖体的鉴定与分离。3. 从异源无细胞蛋白质合成系统中沉淀卵清蛋白多核糖体。

J Biol Chem. 1973 Feb 25;248(4):1424-30.

Biologically and chemically pure mRNA coding for a mouse immunoglobulin L-chain prepared with the aid of antibodies and immobilized oligothymidine.借助抗体和固定化寡聚胸腺嘧啶制备的编码小鼠免疫球蛋白轻链的生物和化学纯mRNA。

Proc Natl Acad Sci U S A. 1973 Aug;70(8):2256-60. doi: 10.1073/pnas.70.8.2256.

Isolation of messenger RNA coding for mouse heavy-chain immunoglobulin.编码小鼠重链免疫球蛋白的信使核糖核酸的分离

Proc Natl Acad Sci U S A. 1973 Apr;70(4):1127-31. doi: 10.1073/pnas.70.4.1127.

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