编码小鼠重链免疫球蛋白的信使核糖核酸的分离
Isolation of messenger RNA coding for mouse heavy-chain immunoglobulin.
作者信息
Stevens R H, Williamson A R
出版信息
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1127-31. doi: 10.1073/pnas.70.4.1127.
A method for purification of the mRNA coding for heavy-chain protein of a mouse G(2a) myeloma (5563) is reported. Complete myeloma protein (H(2)L(2)) specifically binds to the heavy-chain mRNA, and the resultant RNA-protein complex is precipitated with antiserum against 5563 myeloma protein. Cytoplasmic RNA isolated by this method showed two bands of 6.0 x 10(5) and 3.1 x 10(5) molecular weight when analyzed by acrylamide gel electrophoresis. The RNA in the bands contained 1.5% and 8% A-rich regions, respectively. The RNA in each of the bands contained sequences for heavy-chain protein when translated in Xenopus laevis oocytes.
报道了一种纯化小鼠G(2a)骨髓瘤(5563)重链蛋白编码mRNA的方法。完整的骨髓瘤蛋白(H(2)L(2))特异性结合重链mRNA，所得的RNA-蛋白复合物用抗5563骨髓瘤蛋白的抗血清沉淀。用这种方法分离的细胞质RNA经丙烯酰胺凝胶电泳分析显示出两条分子量分别为6.0×10(5)和3.1×10(5)的条带。条带中的RNA分别含有1.5%和8%富含A的区域。当在非洲爪蟾卵母细胞中翻译时，每个条带中的RNA都含有重链蛋白的序列。
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