Short J, Kibert L, Wedmore R, Ove P, Zemel R
Cytobios. 1979;25(98):115-28.
Chromatin protein methylation in proliferating liver cells and hepatomas was examined in vivo and in vitro. Methylation in vivo was estimated using 2-14 C-L-methionine and 3H-methyl-L-methionine. 3H/14C ratios were calculated for crude histone and nonhistone chromatin protein fractions and compared to those calculated for serum albumin. From this, determination of a methylation index was calculated using serum albumin as a standard. Results of this study indicate that both histones and nonhistone chromatin proteins are methylated to a greater extent in proliferating liver cells and in hepatomas than in control preparations. These observations are generally supported by our in vitro methylation studies. The results are discussed in terms of chromatin protein methylation and its possible relationship to DNA replication.
在体内和体外研究了增殖性肝细胞和肝癌细胞中的染色质蛋白甲基化。体内甲基化使用2-¹⁴C-L-甲硫氨酸和³H-甲基-L-甲硫氨酸进行评估。计算粗组蛋白和非组蛋白染色质蛋白组分的³H/¹⁴C比率，并与血清白蛋白的³H/¹⁴C比率进行比较。据此，以血清白蛋白为标准计算甲基化指数。本研究结果表明，与对照制剂相比，增殖性肝细胞和肝癌细胞中的组蛋白和非组蛋白染色质蛋白甲基化程度更高。我们的体外甲基化研究总体上支持了这些观察结果。从染色质蛋白甲基化及其与DNA复制的可能关系方面对结果进行了讨论。
