Davie J R, Delcuve G P, Nickel B E, Moirier R, Bailey G
Department of Biochemistry, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Cancer Res. 1987 Oct 15;47(20):5407-10.
The levels of histone subtypes and DNA methylation of aflatoxin-induced rainbow trout hepatocellular carcinoma and adult liver nuclei were compared. The hepatocellular carcinoma nuclei were enriched in the ubiquitinated species of histone H2A and depleted in histones H1o and H1b. The 5-methylcytosine content and methylation patterns of the vitellogenin genes and the transcriptionally inactive TPG-3 protamine gene were not altered in the trout hepatocellular carcinoma DNA. Thus, undermethylation of DNA is not a general feature of chemically induced tumors in vivo.
比较了黄曲霉毒素诱导的虹鳟肝细胞癌和成年肝脏细胞核中组蛋白亚型水平及DNA甲基化情况。肝细胞癌细胞核中泛素化的组蛋白H2A含量丰富，而组蛋白H1o和H1b含量减少。在虹鳟肝细胞癌DNA中，卵黄蛋白原基因和转录无活性的TPG - 3鱼精蛋白基因的5 - 甲基胞嘧啶含量及甲基化模式未发生改变。因此，DNA低甲基化并非体内化学诱导肿瘤的普遍特征。
