David H L
Appl Microbiol. 1974 Oct;28(4):696-9. doi: 10.1128/am.28.4.696-699.1974.
Partitioned between aqueous methanol and petroleum ether, the unsaponifiable pigments of Mycobacterium kansasii were all epiphasic. Thin-layer chromatography of these carotenoids showed that M. kansasii formed at least nine pigments. These pigments were identified by their chromatographic properties and spectral characteristics as phytoene, zeta-carotene, neurosporene, lycopene, leprotene, gamma-carotene, delta-carotene, alpha-carotene, and beta-carotene. Three additional pigmented spots on thin-layer chromatography found in trace amounts were possibly degradation products of the major carotenoids.
堪萨斯分枝杆菌的不皂化色素经水甲醇和石油醚分配后,均位于上层。这些类胡萝卜素的薄层色谱分析表明,堪萨斯分枝杆菌形成了至少九种色素。通过色谱性质和光谱特征将这些色素鉴定为八氢番茄红素、ζ-胡萝卜素、神经孢菌红素、番茄红素、麻风烯、γ-胡萝卜素、δ-胡萝卜素、α-胡萝卜素和β-胡萝卜素。在薄层色谱上发现的另外三个微量色素斑点可能是主要类胡萝卜素的降解产物。