Mittwoch U, Kirk D J, Wilkie D
J Med Genet. 1974 Sep;11(3):260-6. doi: 10.1136/jmg.11.3.260.
Chloramphenicol, at concentrations of 500 μg/ml, had a marked inhibitory effect on mitoses in human fibroblasts. Metaphase and G2 appeared to be particularly sensitive to the action of the drug. With concentrations of 1000 μg/ml, or with 500 μg/ml after prolonged exposure, the cells did not reach G2 and no mitoses were seen. The drug required several hours to produce its effect. Studies of oxygen uptake showed no immediate reduction in the respiratory activity of cells at concentrations of 500 μg/ml or 1000 μg/ml but cells which had been in contact with 500 μg/ml of drug for 24 hours showed a marked reduction in respiratory activity. Since the primary effect of chloramphenicol is arrest of mitochondrial protein synthesis, it is concluded that continued synthesis of mitochondrial proteins in the maintenance of the respiratory chain are prerequisites for normal cell division in fibroblasts.
氯霉素在浓度为500微克/毫升时，对人成纤维细胞的有丝分裂有显著抑制作用。中期和G2期似乎对该药物的作用特别敏感。在浓度为1000微克/毫升时，或长时间暴露于500微克/毫升后，细胞无法进入G2期，也未见有丝分裂。该药物需要数小时才能产生作用。对氧摄取的研究表明，在浓度为500微克/毫升或1000微克/毫升时，细胞的呼吸活性没有立即降低，但与500微克/毫升药物接触24小时的细胞显示呼吸活性显著降低。由于氯霉素的主要作用是阻止线粒体蛋白质合成，因此可以得出结论，线粒体蛋白质的持续合成以维持呼吸链是成纤维细胞正常细胞分裂的先决条件。
