Mittwoch U, Wilkie D
Br J Exp Pathol. 1971 Apr;52(2):186-91.
DNA values of fibroblast-type cells were measured by Feulgen microdensitometry. Cells cultured for periods ranging from 18-48 hr in the presence of 20 μg./ml. chlorimipramine were found to be mainly in the G1 phase and significantly fewer cells were seen in S and G2 phases than in untreated cultures. This indicated that the drug inhibits the initiation of DNA synthesis, a conclusion supported by the cytological findings in which significantly fewer treated cells were seen in prophase of mitosis compared with control cultures. Also fewer treated cells were in anaphase and telophase but significantly more metaphases were seen than in controls. It is concluded that the drug inhibits initiation of mitotic division due, at least partly, to blockage of DNA synthesis, but cells having entered mitosis are arrested at metaphase. There was evidence of recovery after 3 hr treatment with the drug. The results suggested that the addition of ATP to the medium may enhance recovery.
通过福尔根显微密度测定法测量成纤维细胞型细胞的DNA值。在存在20μg/ml氯米帕明的情况下培养18至48小时的细胞主要处于G1期，与未处理的培养物相比，S期和G2期的细胞明显较少。这表明该药物抑制DNA合成的起始，这一结论得到细胞学结果的支持，即与对照培养物相比，在有丝分裂前期观察到的经处理细胞明显较少。处于后期和末期的处理细胞也较少，但与对照相比，中期的细胞明显更多。得出的结论是，该药物至少部分由于DNA合成受阻而抑制有丝分裂的起始，但进入有丝分裂的细胞在中期停滞。有证据表明用该药物处理3小时后有恢复。结果表明向培养基中添加ATP可能会增强恢复。
