抗血清亲和力对抑制反应的影响。
Influence of antiserum avidity on inhibition reactions.
作者信息
Tosi R M, Celada F
出版信息
Immunology. 1974 Nov;27(5):941-51.
The presence in each antiserum of different antibody components, with different binding affinities, quantitatively affects the outcome of an inhibition reaction. A detailed analysis permits the recognition of the following conditions. (a) At high antigen concentrations, the antiserum is approaching saturation; the only effect of adding unlabelled antigen is competition with the labelled antigen for the available antibody sites. (b) At intermediate antigen concentrations, only a fraction of the antibody components, those of higher affinity, are contributing to the binding reaction; in addition to competition, unlabelled antigen produces an increase in the number of reacting antibody sites. The resultant of these two counteracting effects can be predicted according to the slope of a simple linear function. (c) Lastly, at low antigen concentrations, the added antigen does not have any significant inhibitory effect. As a result of this analysis, practical indications are available for selecting antisera for radioimmunoassays on the basis of their avidity parameters, for choosing the most suitable antigen range for each antiserum, and for interpreting the shape of inhibition curves in terms of the avidity and heterogeneity of the antiserum.
每种抗血清中存在具有不同结合亲和力的不同抗体成分，这在数量上会影响抑制反应的结果。详细分析可识别以下情况。(a) 在高抗原浓度下，抗血清接近饱和；添加未标记抗原的唯一作用是与标记抗原竞争可用的抗体位点。(b) 在中等抗原浓度下，只有一部分抗体成分，即那些具有较高亲和力的成分，参与结合反应；除了竞争外，未标记抗原还会使反应性抗体位点数量增加。这两种抵消作用的结果可以根据一个简单线性函数的斜率来预测。(c) 最后，在低抗原浓度下，添加的抗原没有任何显著的抑制作用。基于这一分析，可获得一些实际指导，用于根据抗血清的亲和力参数选择用于放射免疫分析的抗血清，为每种抗血清选择最合适的抗原范围，以及根据抗血清的亲和力和异质性来解释抑制曲线的形状。
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