Haars E G, Schmidt J M
J Bacteriol. 1974 Dec;120(3):1409-16. doi: 10.1128/jb.120.3.1409-1416.1974.
Estimates of average rates of stalk formation over several generations of growth in Caulobacter crescentus showed that long-stalked Sk1 mutant and phosphate-starved wild-type cultures produce stalk material at about twice the rate of wild-type C. crescentus grown with adequate nutrients. Thus, the long stalks of Sk1 or phosphate-starved caulobacters are not merely a function of their longer doubling times. Inhibition of cell division of Sk1 418 with mitomycin C (MC) caused production of cellular filaments and resulted in inhibition of stalk formation. There was no appreciable decrease in total cell mass or in rates of ribonucleic acid and protein synthesis in the MC-treated cultures as compared with controls, but stalk formation, which is normally dependent on these processes, was severely retarded. Average stalk lengths in MC-treated Sk1 cultures were 30% of those found in control cultures. MC-produced cellular filaments were also subjected to deoxyribonucleic acid analysis and ultrastructural examination. The deoxyribonucleic acid content of MC-treated bacteria was about 50 to 60% that of untreated bacteria. Hydroxyurea also was found to produce some cellular filaments and shorter stalks, but with accompanying decreases in growth rate and yield.
对新月柄杆菌几代生长过程中柄形成平均速率的估计表明，长柄Sk1突变体和缺磷野生型培养物产生柄物质的速率约为营养充足条件下生长的野生型新月柄杆菌的两倍。因此，Sk1或缺磷柄杆菌的长柄不仅仅是其较长倍增时间的函数。用丝裂霉素C（MC）抑制Sk1 418的细胞分裂会导致细胞形成丝状，并抑制柄的形成。与对照相比，经MC处理的培养物中细胞总质量以及核糖核酸和蛋白质合成速率没有明显下降，但通常依赖于这些过程的柄形成受到严重阻碍。经MC处理的Sk1培养物中的平均柄长度为对照培养物中平均柄长度的30%。对MC产生的细胞丝状结构也进行了脱氧核糖核酸分析和超微结构检查。经MC处理的细菌的脱氧核糖核酸含量约为未处理细菌的50%至60%。还发现羟基脲会产生一些细胞丝状结构和较短的柄，但同时伴随着生长速率和产量的下降。
