Terrana B, Newton A
J Bacteriol. 1976 Oct;128(1):456-62. doi: 10.1128/jb.128.1.456-462.1976.
Penicillin G at low concentrations blocked cell division in Caulobacter crescentus without inhibiting cell growth. The long filamentous cells formed after two to three generations under these conditions had a stalk at one pole and usually one or more flagella at the opposite pole. The failure of the filaments to form a second stalk at the flagellated pole indicates that stalk formation was dependent upon completion of a step that was also required for cell division. Two observations support this conclusion. (i) Penicillin did not stop the normal development of synchronous swarmer cells into stalked initiation and stalk elongation. (ii) When the action of penicillin was reversed by the addition of penicillinase to cultures of filaments, stalks were not formed at the nonstalked pole until after cell division had occurred; thus the normal order of development events was maintained: cell division leads to stalk formation. These results are consistent with a model in which the organization of the developmental program for stalk formation occurs before cell division as a consequence of steps that branch from the cell division pathway.
低浓度的青霉素G可阻断新月柄杆菌的细胞分裂，而不抑制细胞生长。在这些条件下经过两到三代后形成的长丝状细胞，一端有柄，另一端通常有一根或多根鞭毛。丝状细胞在有鞭毛的一端未能形成第二个柄，这表明柄的形成依赖于细胞分裂所需步骤的完成。两项观察结果支持这一结论。（i）青霉素不会阻止同步游动细胞正常发育为有柄起始细胞和柄伸长。（ii）当向丝状细胞培养物中添加青霉素酶使青霉素的作用逆转时，直到细胞分裂发生后，无柄的一端才会形成柄；因此，发育事件的正常顺序得以维持：细胞分裂导致柄的形成。这些结果与一个模型一致，即在细胞分裂之前，由于从细胞分裂途径分支出来的步骤，形成柄的发育程序得以组织起来。
