普拉特链霉菌对麦角腈的N-去甲基化作用。
N-demethylation of lergotrile by Streptomyces platensis.
作者信息
Davis P J, Glade J C, Clark A M, Smith R V
出版信息
Appl Environ Microbiol. 1979 Nov;38(5):891-3. doi: 10.1128/aem.38.5.891-893.1979.
Thirty-eight microorganisms were screened for their ability to produce metabolites of the semisynthetic alkaloid, lergotrile. A total of five microorganisms were found to biotransform lergotrile, and N-desmethyl lergotrile was detected as the principal metabolite with most organisms. Streptomyces platensis (NRRL 2364) appeared to form the metabolite in highest yield, and a preparative-scale conversion was accomplished with a recovered yield of 50%. Structure proof was accomplished with comparative thin-layer chromatography, mixed melting point, mass spectrometry, and remethylation to lergotrile.
对38种微生物进行了筛选，以评估它们产生半合成生物碱麦角腈代谢物的能力。共发现5种微生物可生物转化麦角腈，大多数微生物产生的主要代谢物为N-去甲基麦角腈。普拉特链霉菌（NRRL 2364）似乎能以最高产量形成该代谢物，通过制备规模的转化获得了50%的回收率。通过比较薄层色谱、混合熔点、质谱分析以及将其重新甲基化生成麦角腈来完成结构鉴定。
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