Singer J W, Doney K C, Thomas E D
Blood. 1979 Jul;54(1):180-5.
We studied the effects of peripheral blood lymphocytes from 16 untransfused patients with severe aplastic anemia (AA) of diverse etiologies on the growth of granulocyte-macrophage colonies from normal marrows. Normal lymphocytes in our system increased the number of granulocytic colonies by 31 +/- 6% (mean +/- SEM). Lymphocytes from 3 of 16 untransfused AA patients significantly inhibited growth in HLA-matched sibling marrows (-30%, -40%, and -37%; p less than 0.01). Although these results suggest that the majority of cases of AA are not mediated by a coculture-detectable immunologic mechanism, studies using lymphocytes obtained from AA patients before transfusions may detect the subpopulation whose disease is immune-mediated and who may therefore respond to immunosuppressive therapy.
我们研究了16例病因各异的未经输血的重型再生障碍性贫血（AA）患者的外周血淋巴细胞对正常骨髓中粒细胞-巨噬细胞集落生长的影响。在我们的系统中，正常淋巴细胞使粒细胞集落数量增加了31±6%（平均值±标准误）。16例未经输血的AA患者中有3例的淋巴细胞显著抑制了HLA匹配的同胞骨髓的生长（分别为-30%、-40%和-37%；p<0.01）。尽管这些结果表明大多数AA病例不是由共培养可检测的免疫机制介导的，但使用输血前从AA患者获得的淋巴细胞进行的研究可能会检测到疾病由免疫介导且因此可能对免疫抑制治疗有反应的亚群。
