Groscurth P, Cheng S, Vollenweider I, von Felten A
Acta Haematol. 1987;77(3):150-5. doi: 10.1159/000205980.
The ultrastructure of washed and gel-filtered platelets has been examined by scanning- and transmission-electron microscopy. Three different washing procedures previously established for functional studies have been employed. Each washing method resulted in typical morphologic alterations of the platelets. Gel filtration of platelet-rich plasma appeared less harmful than washing. Specimens fixed immediately after elution contained numerous platelets with typical shape changes which were restored almost completely after incubation at 37 degrees C. Our study emphasizes the need for cautious interpretation of results obtained with separated platelets and mandates morphologic controls for such experiments.
通过扫描电子显微镜和透射电子显微镜对洗涤和凝胶过滤后的血小板超微结构进行了检查。采用了先前为功能研究建立的三种不同洗涤程序。每种洗涤方法都导致血小板出现典型的形态学改变。富含血小板血浆的凝胶过滤似乎比洗涤的危害小。洗脱后立即固定的标本含有许多具有典型形状变化的血小板，在37℃孵育后几乎完全恢复。我们的研究强调，对于分离血小板所获得的结果需要谨慎解读，并要求对此类实验进行形态学对照。
