猪肝丙酮酸羧化酶。丙酮酸羧化反应途径。
Pig liver pyruvate carboxylase. The reaction pathway for the carboxylation of pyruvate.
作者信息
Warren G B, Tipton K F
出版信息
Biochem J. 1974 May;139(2):311-20. doi: 10.1042/bj1390311.
Abstract
- The reaction pathway for the carboxylation of pyruvate, catalysed by pig liver pyruvate carboxylase, was studied in the presence of saturating concentrations of K(+) and acetyl-CoA. 2. Free Mg(2+) binds to the enzyme in an equilibrium fashion and remains bound during all further catalytic cycles. MgATP(2-) binds next, followed by HCO(3) (-) and then pyruvate. Oxaloacetate is released before the random release, at equilibrium, of P(i) and MgADP(-). 3. This reaction pathway is compared with the double displacement (Ping Pong) mechanisms that have previously been described for pyruvate carboxylases from other sources. The reaction pathway proposed for the pig liver enzyme is superior in that it shows no kinetic inconsistencies and satisfactorily explains the low rate of the ATP[unk][(32)P]P(i) equilibrium exchange reaction. 4. Values are presented for the stability constants of the magnesium complexes of ATP, ADP, acetyl-CoA, P(i), pyruvate and oxaloacetate.
摘要
- 在饱和浓度的K⁺和乙酰辅酶A存在的情况下，研究了猪肝丙酮酸羧化酶催化丙酮酸羧化的反应途径。2. 游离的Mg²⁺以平衡方式与酶结合，并在所有后续催化循环中保持结合状态。接下来MgATP²⁻结合，然后是HCO₃⁻，再然后是丙酮酸。草酰乙酸在P₁和MgADP⁻在平衡时随机释放之前被释放。3. 将此反应途径与先前描述的来自其他来源的丙酮酸羧化酶的双置换（乒乓）机制进行了比较。所提出的猪肝酶的反应途径更具优势，因为它没有动力学上的不一致之处，并且令人满意地解释了ATP[未标记][³²P]P₁平衡交换反应的低速率。4. 给出了ATP、ADP、乙酰辅酶A、P₁、丙酮酸和草酰乙酸的镁配合物的稳定常数的值。
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本文引用的文献
1
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J Biol Chem. 1965 Feb;240:574-81.
2
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Biochemistry. 1964 Jan;3:18-26. doi: 10.1021/bi00889a005.
3
PYRUVATE CARBOXYLASE. II. PROPERTIES.丙酮酸羧化酶。II. 性质。
J Biol Chem. 1963 Aug;238:2609-14.
4
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J Biochem. 1963 Jul;54:17-24. doi: 10.1093/oxfordjournals.jbchem.a127741.
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