Chin K N, Hudson G
Br J Exp Pathol. 1974 Dec;55(6):554-61.
Peritoneal cells were studied at intervals of between 6 h and 30 days following a single intravenous injection of a sublethal dose of cyclophosphamide. With the electron microscope, evidence of cell damage and death could be seen at 6 h, and by 12 h large numbers of dead cells were noted, either lying free or within the cytoplasm of macrophages. Most of the damaged cells were lymphocytes but degenerating blast cells, eosinophils, neutrophils and mast cells were also identified. Nuclei were seen in which margination of chromatin had occurred but nuclei of uniform density were also prominent and showed irregular shape and lobulation. Macrophages exhibited all stages of phagocytosis and digestion and a few phagocytes of atypical appearance were noted. By 24 h most of the dead cells lay within the cytoplasm of macrophages which showed many phagocytic inclusions as well as lipid droplets. By 6 days, the peritoneal cells had regained normal appearances although the proportion of lymphoid cells was still reduced. By the 18th day, all features were indistinguishable from normal. The changes observed showed a general similarity to those noted previously in the lymphoreticular cells of the Peyer's patch; they provide no evidence that the environment of the peritoneal cavity protects cells against the action of cyclophosphamide.
在单次静脉注射亚致死剂量的环磷酰胺后，每隔6小时至30天对腹膜细胞进行研究。用电子显微镜观察，在6小时时可见细胞损伤和死亡的迹象，到12小时时，发现大量死亡细胞，它们要么游离存在，要么存在于巨噬细胞的细胞质内。大多数受损细胞是淋巴细胞，但也鉴定出了退化的母细胞、嗜酸性粒细胞、中性粒细胞和肥大细胞。可见到染色质边缘化的细胞核，但密度均匀的细胞核也很突出，且呈现出不规则形状和分叶。巨噬细胞呈现出吞噬和消化的各个阶段，并注意到一些外观不典型的吞噬细胞。到24小时时，大多数死亡细胞存在于巨噬细胞的细胞质内，巨噬细胞显示出许多吞噬内含物以及脂滴。到6天时，腹膜细胞恢复了正常外观，尽管淋巴细胞的比例仍然降低。到第18天时，所有特征与正常情况无法区分。观察到的这些变化与先前在派尔集合淋巴结的淋巴网状细胞中所观察到的变化总体相似；它们没有提供证据表明腹膜腔环境能保护细胞免受环磷酰胺的作用。
