Chin K N, Hudson G
Br J Exp Pathol. 1970 Dec;51(6):563-9.
An electron microscope study has been made of the Peyer's patches of mice at intervals of between 6 hr and 30 days following a single sublethal injection of cyclophosphamide. Evidence of cellular damage and death was already present in some lymphocytes of the Peyer's patch at 6 hr. An early morphological change was the margination of dense chromatin at the nuclear membrane, with a rather sharp inner border. By 12 hr, large numbers of dead cells were present in the lymphoid tissue, either lying free or within the cytoplasm of macrophages. At 24 hr most of the dead cells and cellular remains were seen to lie within the cytoplasm of macrophages and the lymphoid tissue appeared less cellular than that of the controls. Apparently normal lymphocytes, plasma cells, eosinophils and reticular cells were still present, as well as large binucleate cells which were not seen in the controls. At later intervals, the profiles of the macrophage inclusions appeared smaller. By the 9th day, there was an apparent increase in the cellularity of the lymphoid tissue, and immature forms and cells in mitosis were seen more frequently. By the 18th day, the morphology of the Peyer's patch was indistinguishable from normal.
对单次亚致死剂量注射环磷酰胺后的小鼠派伊尔结进行了电子显微镜研究，时间间隔为6小时至30天。在6小时时，派伊尔结的一些淋巴细胞中已出现细胞损伤和死亡的迹象。早期的形态学变化是核膜处致密染色质边缘化，内边界相当清晰。到12小时时，淋巴组织中有大量死亡细胞，它们要么游离存在，要么存在于巨噬细胞的细胞质中。到24小时时，大部分死亡细胞和细胞残骸位于巨噬细胞的细胞质中，淋巴组织中的细胞数量似乎比对照组少。明显正常的淋巴细胞、浆细胞、嗜酸性粒细胞和网状细胞仍然存在，还有对照组中未见的大的双核细胞。在之后的时间间隔里，巨噬细胞内含物的轮廓看起来变小了。到第9天时，淋巴组织的细胞数量明显增加，未成熟形式的细胞和处于有丝分裂期的细胞更频繁地出现。到第18天时，派伊尔结的形态与正常情况无法区分。
