选择性蛋白水解对完整大鼠血小板积累5-羟色胺的影响。
Effect of selective proteolysis on the accumulation of 5-hydroxytryptamine by intact rat blood platelets.
作者信息
Sneddon J M, Williams K I
出版信息
Br J Pharmacol. 1974 Oct;52(2):237-44. doi: 10.1111/j.1476-5381.1974.tb09706.x.
1 The effect of perturbation of intact blood platelets with proteolytic enzymes was studied with respect to 5-hydroxytryptamine (5-HT) transport, adenine transport and intracellular Na(+) and K(+) levels.2 Leucine aminopeptidase and thrombin reduced 5-HT transport, released 5-HT from pre-labelled platelets and disturbed the gradient to monovalent cations. Leucine amino-peptidase, but not thrombin, inhibited adenine transport.3 alpha-Chymotrypsin and carboxypeptidases A and B were without effect on the parameters studied.4 Trypsin selectively reduced 5-HT transport. It did not release 5-HT from blood platelets or inhibit adenine transport.5 The action of proteolytic enzymes is discussed in terms of proteins localized in the external membrane that may function in part as membrane carriers.
研究了蛋白水解酶对完整血小板的扰动作用，涉及5-羟色胺（5-HT）转运、腺嘌呤转运以及细胞内钠（Na⁺）和钾（K⁺）水平。
亮氨酸氨肽酶和凝血酶降低了5-HT转运，从预先标记的血小板中释放了5-HT，并扰乱了单价阳离子梯度。亮氨酸氨肽酶而非凝血酶抑制了腺嘌呤转运。
α-胰凝乳蛋白酶以及羧肽酶A和B对所研究的参数没有影响。
胰蛋白酶选择性地降低了5-HT转运。它不会从血小板中释放5-HT，也不会抑制腺嘌呤转运。
根据位于外膜的蛋白质来讨论蛋白水解酶的作用，这些蛋白质可能部分作为膜载体发挥功能。
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