在受刺激的人类淋巴细胞中DNA酶诱导出现两波的证据。
Evidence for two waves of induction of DNA enzymes in stimulated human lymphocytes.
作者信息
Noy G C, Dalpra L, Pedrini A M, Ciarrocchi G, Giulotto E, Nuzzo F, Falaschi A
出版信息
Nucleic Acids Res. 1974 Sep;1(9):1183-99. doi: 10.1093/nar/1.9.1183.
Abstract
The stimulation of human lymphocytes with phytohaemoagglutinin induces the appearance or increase of several enzymes of DNA metabolism [Pedrini etal., Biochem. Biophys. Res. Comm., 47:1221(1972)]. With long times of stimulation, two phenomena are observed; an increase in the levels of DNA polymerase, of a DNase acting on single-stranded DNA, and of an endonuclease, occurring between the third and fourth day, in parallel with a wave of DNA synthesis;a second wave of increase of the same enzymes and of DNA ligase,occurring between the fifth and eight day when the DNA replication rate, as measured by thymidine-pulses, has decreased to values close to the background.
摘要
用植物血球凝集素刺激人淋巴细胞会诱导几种DNA代谢酶的出现或增加[佩德里尼等人，《生物化学与生物物理研究通讯》，47:1221(1972)]。长时间刺激后，会观察到两种现象：在第三天到第四天之间，DNA聚合酶、作用于单链DNA的脱氧核糖核酸酶以及核酸内切酶的水平增加，这与一波DNA合成同时发生；在第五天到第八天之间，当通过胸苷脉冲测量的DNA复制速率降至接近背景值时，相同的酶以及DNA连接酶出现第二波增加。
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