Collins F M, Woodruff W H
Zentralbl Bakteriol Orig A. 1979 Oct;245(1-2):106-13.
Normal ICR mice were injected intraperitoneally with 0.5 ml of thioglycollate broth or 5 x 10(8) heat-killed Pasteurella multocida vaccine and the number of polymorphs, lymphocytes and macrophages in the peritoneal washout suspensions were determined at intervals up to 72 hours. The stimulated mice were challenged intraperitoneally with opsonized or unopsonized P. multocida at increasing time intervals and the rate of growth by the organisms in the washout suspension was determined up to 60 minutes later. The opsonized bacilli were taken up by the 6 hr. exudate cells (50-60% PMNs) and their growth inhibited more effectively than when the 72 hr exudate cells were tested (only 10% PMNs). When the challenge inoculum was introduced into the peritoneal cavities of mice stimulated 6 hrs previously with 5 x 10(8) heat-killed P. multocida vaccine, up to 80% of the bacilli were inactivated over a 30 minute period. However, when 72 or 250 hr peritoneal exudate cells were tested, the inoculum was not inactivated, but showed an increasingly lethal effect.
将0.5毫升硫乙醇酸盐肉汤或5×10⁸热灭活多杀巴斯德菌疫苗腹腔注射到正常ICR小鼠体内，在长达72小时的时间间隔内测定腹腔冲洗液悬浮液中多形核细胞、淋巴细胞和巨噬细胞的数量。在不同时间间隔对受刺激的小鼠进行腹腔注射调理或未调理的多杀巴斯德菌攻击，在60分钟后测定冲洗液悬浮液中细菌的生长速率。调理后的杆菌被6小时的渗出细胞（50 - 60%为多形核白细胞）摄取，其生长抑制效果比测试72小时的渗出细胞时更有效（仅10%为多形核白细胞）。当将攻击接种物引入6小时前用5×10⁸热灭活多杀巴斯德菌疫苗刺激过的小鼠腹腔时，在30分钟内高达80%的杆菌被灭活。然而，当测试72小时或250小时的腹腔渗出细胞时，接种物未被灭活，反而显示出越来越强的致死作用。
