Campbell S G, Mtero S S, Niderbuhl C J, Collins F M
Infect Immun. 1983 Jan;39(1):202-7. doi: 10.1128/iai.39.1.202-207.1983.
When Pasteurella hemolytica was introduced into conventionally raised ICR mice by a variety of routes (intraperitoneal, aerogenic, and oral), the inoculum was rapidly eliminated, and none of the mice died. Even when the inoculum was injected intraperitoneally into sublethally irradiated (600 rads) mice, the organisms were eliminated rapidly unless suspended in 10% hog gastric mucin. When germfree ICR mice were orally infected with P. hemolytica, the infection established itself in the intestinal tract and spread to the mesenteric lymph nodes but did not progress beyond this point. Despite the inability of P. hemolytica to establish itself systemically, the organism multiplied freely in mouse blood and a homogenate of normal mouse lung in vitro. Normal mouse peritoneal macrophages could phagocytose P. hemolytica in vitro, although not as efficiently as the control Listeria monocytogenes suspensions. The addition of hyperimmune bovine serum (opsonin) to the P. hemolytica suspension increased phagocytosis but did not greatly affect the subsequent bactericidal activity of the macrophages in vitro. The reason for the lack of pathogenicity shown by P. hemolytica in normal mice remains enigmatic.
当通过多种途径（腹腔内、气源性和口服）将溶血巴斯德菌引入常规饲养的ICR小鼠时，接种物迅速被清除，没有小鼠死亡。即使将接种物腹腔内注射到亚致死剂量照射（600拉德）的小鼠体内，除非悬浮在10%的猪胃粘蛋白中，否则微生物也会迅速被清除。当无菌ICR小鼠经口感染溶血巴斯德菌时，感染在肠道内建立并扩散到肠系膜淋巴结，但未超过此范围。尽管溶血巴斯德菌无法在全身定植，但该菌在体外能在小鼠血液和正常小鼠肺匀浆中自由繁殖。正常小鼠腹腔巨噬细胞在体外可吞噬溶血巴斯德菌，尽管效率不如对照单核细胞增生李斯特菌悬液。向溶血巴斯德菌悬液中添加超免疫牛血清（调理素）可增强吞噬作用，但对巨噬细胞随后的体外杀菌活性影响不大。溶血巴斯德菌在正常小鼠中缺乏致病性的原因仍然不明。
