菊芋组织中的核糖核酸聚合酶活性。
Ribonucleic acid polymerase activities in Jerusalem artichoke tissue.
作者信息
Gore J R, Ingle J
出版信息
Biochem J. 1974 Oct;143(1):107-113. doi: 10.1042/bj1430107.
Abstract
- Artichoke tuber tissue contained RNA polymerase activity bound to the chromatin and in the supernatant after chromatin sedimentation. 2. The activity in the supernatant, the soluble polymerase, was fractionated into polymerases I and II by DEAE-cellulose chromatography, and the properties of each activity were determined. 3. The proportions of chromatin-bound and soluble activities varied with growth of the tissue, and there was a correlation between chromatin-bound activity and RNA accumulation. 4. The properties of the solubilized chromatin activity were compared with those of the soluble activity, and the relationship between these two activities is discussed.
摘要
- 洋蓟块茎组织含有与染色质结合的RNA聚合酶活性以及染色质沉降后上清液中的该活性。2. 上清液中的活性，即可溶性聚合酶，通过DEAE - 纤维素色谱法分离为聚合酶I和聚合酶II，并测定了每种活性的特性。3. 与染色质结合的活性和可溶性活性的比例随组织生长而变化，且与染色质结合活性和RNA积累之间存在相关性。4. 将溶解的染色质活性的特性与可溶性活性的特性进行了比较，并讨论了这两种活性之间的关系。
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