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1
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Proc Natl Acad Sci U S A. 1974 Jul;71(7):2740-3. doi: 10.1073/pnas.71.7.2740.
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Transcription of chromatin. Initial and terminal nucleotides of ribonucleic acid synthesized by calf thymus and Escherichia coli ribonucleic acid polymerases.染色质的转录。小牛胸腺和大肠杆菌核糖核酸聚合酶合成的核糖核酸的起始和末端核苷酸。
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Transcription of fractionated calf thymus chromatin by RNA polymerase of calf thymus and Escherichia coli.用小牛胸腺和大肠杆菌的RNA聚合酶对分级分离的小牛胸腺染色质进行转录。
Biochemistry. 1975 Oct 21;14(21):4764-71. doi: 10.1021/bi00692a031.
4
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Proc Natl Acad Sci U S A. 1973 May;70(5):1326-30. doi: 10.1073/pnas.70.5.1326.
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6
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Transcription of DNA and chromatin with calf thymus RNA polymerase B in vitro.体外使用小牛胸腺RNA聚合酶B进行DNA和染色质的转录。
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10
Enhancement of template activity of calf thymus and rat liver chromatin by -iirradiation.通过γ射线辐照增强小牛胸腺和大鼠肝脏染色质的模板活性。
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引用本文的文献

1
Chemical and physical properties of fractionated chromatin.分级分离染色质的化学和物理性质。
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3328-32. doi: 10.1073/pnas.72.9.3328.
2
Oestradiol-receptor complexes in subnuclear fractions of rat uterine tissue.大鼠子宫组织亚核组分中的雌二醇受体复合物
Nucleic Acids Res. 1978 Jan;5(1):87-103. doi: 10.1093/nar/5.1.87.
3
Sequence specific interaction of the chromosomal proteins with DNA.染色体蛋白与DNA的序列特异性相互作用。
Nucleic Acids Res. 1977 Oct;4(10):3415-39. doi: 10.1093/nar/4.10.3415.
4
Isolation and properties of a thermostable restriction endonuclease (ENDO R-Bst1503).一种热稳定限制性内切核酸酶（ENDO R-Bst1503）的分离与特性
J Bacteriol. 1977 Feb;129(2):1110-20. doi: 10.1128/jb.129.2.1110-1120.1977.

本文引用的文献

1
REPRESSED AND ACTIVE CHROMATIN ISOLATED FROM INTERPHASE LYMPHOCYTES.从间期淋巴细胞中分离出的抑制性染色质和活性染色质。
Proc Natl Acad Sci U S A. 1963 Dec;50(6):1026-32. doi: 10.1073/pnas.50.6.1026.
2
A study of the structure of isolated chromatin.一项关于分离染色质结构的研究。
Biochemistry. 1968 Dec;7(12):4380-8. doi: 10.1021/bi00852a034.
3
Kinetics of the in vitro inhibition of transcription by actinomycin.放线菌素对转录的体外抑制动力学
J Mol Biol. 1970 Jun 14;50(2):421-38. doi: 10.1016/0022-2836(70)90202-0.
4
Fractionation of liver chromatin.肝脏染色质的分级分离
Proc Natl Acad Sci U S A. 1971 Dec;68(12):2941-4. doi: 10.1073/pnas.68.12.2941.
5
Protein-DNA interactions in extended and condensed chromatin.
Biochem Biophys Res Commun. 1973 Dec 19;55(4):1078-84. doi: 10.1016/s0006-291x(73)80005-1.
6
A comparison of the proteins of condensed and extended chromatin fractions of rabbit liver and calf thymus.兔肝和小牛胸腺浓缩染色质组分与伸展染色质组分蛋白质的比较。
Biochemistry. 1973 Sep 25;12(20):3853-8. doi: 10.1021/bi00744a009.
7
Circular dichroism spectra of putative transcribed and repressed chromatin.
Biochem Biophys Res Commun. 1973 May 1;52(1):202-7. doi: 10.1016/0006-291x(73)90974-1.
8
Transcription of chromatin in vitro.体外染色质转录
J Mol Biol. 1973 Jun 25;77(2):237-54. doi: 10.1016/0022-2836(73)90334-3.
9
Fractionation of mouse myeloma chromatin.小鼠骨髓瘤染色质的分级分离
Biochemistry. 1973 Sep 25;12(20):3843-53. doi: 10.1021/bi00744a008.
10
Resolution of a spectrum of nucleoprotein species in sonicated chromatin.超声破碎染色质中一系列核蛋白种类的解析
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2317-21. doi: 10.1073/pnas.69.8.2317.

RNA聚合酶结合位点在分级分离染色质中的分布

Distribution of RNA polymerase binding sites in fractionated chromatin.

作者信息

Simpson R T

出版信息

Proc Natl Acad Sci U S A. 1974 Jul;71(7):2740-3. doi: 10.1073/pnas.71.7.2740.

DOI:10.1073/pnas.71.7.2740
PMID:4604338
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC388545/
Abstract

Calf-thymus chromatin was fractionated by ion-exchange chromatography on ECTHAM-cellulose and sucrose gradient sedimentation. [ECTHAM-cellulose is a cationic adsorbent prepared by coupling tris(hydroxymethyl)aminomethane to cellulose with epichlorohydrin.] The capacity of these fractionated chromatins to support RNA synthesis by DNA-dependent RNA polymerase of Escherichia coli was examined, using procedures that permit measurement of binding site frequency. Unfractionated calf thymus chromatin has 5-10% as many binding sites as protein-free DNA. By combination of the two fractionation methods, chromatin samples were obtained containing as few as 2% and as many as 47% of the number of binding sites found on protein-free DNA.

摘要

小牛胸腺染色质通过在ECTHAM-纤维素上进行离子交换色谱和蔗糖梯度沉降进行分级分离。[ECTHAM-纤维素是一种阳离子吸附剂，通过用环氧氯丙烷将三（羟甲基）氨基甲烷偶联到纤维素上制备而成。] 使用允许测量结合位点频率的方法，检测了这些分级分离的染色质支持大肠杆菌依赖DNA的RNA聚合酶进行RNA合成的能力。未分级分离的小牛胸腺染色质的结合位点数量是无蛋白质DNA的5%-10%。通过将两种分级分离方法相结合，获得了染色质样品，其结合位点数量少至无蛋白质DNA上发现的结合位点数量的2%，多至47%。