Atkins E D, Nieduszynski I A, Horner A A
Biochem J. 1974 Oct;143(1):251-2. doi: 10.1042/bj1430251.
X-ray fibre-diffraction photographs were obtained from oriented films of the sodium salt of macromolecular heparin (molecular weight approx. 10(6)) prepared from rat skin. Two distinct molecular chain conformations corresponding to two different crystal lattices were observed as a function of relative humidity. The first conformation, obtained at 78% relative humidity, has a layer-line spacing of 1.73nm, which can be interpreted as an approximate twofold helix. On increasing the relative humidity to 84% a second phase with a layer-line repeat of 1.65nm is obtained with the reflexions indexing on a triclinic unit cell similar to that obtained previously (Nieduszynski & Atkins, 1973) for pig mucosal heparin.
从大鼠皮肤制备的大分子肝素钠盐（分子量约为10⁶）的取向薄膜获得了X射线纤维衍射照片。观察到对应于两种不同晶格的两种不同分子链构象随相对湿度而变化。在78%相对湿度下获得的第一种构象，其层线间距为1.73nm，可解释为近似的双螺旋。将相对湿度提高到84%时，获得了第二种相，其层线重复间距为1.65nm，其反射在与先前（Nieduszynski和Atkins，1973年）为猪黏膜肝素获得的三斜晶胞上进行指标化。
