小鼠芳基硫酸酯酶C的品系间变异

Interstrain variation of murine arylsulfatase C.

作者信息

Nelson K, Daniel W L

出版信息

Experientia. 1979 Mar 15;35(3):309-10. doi: 10.1007/BF01964317.

DOI:10.1007/BF01964317

Abstract

A method has been developed for the assay of arylsulfatase C in tissue extracts containing arylsulfatases A and B. Significant variation of enzyme activity was observed among 26 inbred murine strains. Activity differences were apparent at all stages evaluated between 1 and 70 days postnatal age. Arylsulfatase C from representative high- and low-activity strains exhibited similar Michaelis constants, temperature optima, pH optima, thermostabilities and inhibitor profiles.

摘要

已开发出一种用于检测含有芳基硫酸酯酶A和B的组织提取物中芳基硫酸酯酶C的方法。在26个近交系小鼠品系中观察到酶活性存在显著差异。在出生后1至70天评估的所有阶段，活性差异均很明显。来自代表性高活性和低活性品系的芳基硫酸酯酶C表现出相似的米氏常数、最适温度、最适pH值、热稳定性和抑制剂谱。