Mori M, Arimitsu Y, Otani S, Akama K
Jpn J Med Sci Biol. 1974 Dec;27(6):297-308. doi: 10.7883/yoken1952.27.297.
Experimental leptospirosis with Leptospira icterohaemorrhagiae Shibaura strain was studied in guinea pigs. When the pathogen was inoculated intracutaneously to the back of the animals, localized haemorrhage was observed at the inoculated site before the appearance of general haemorrhage. The severity of the local lesion increased progressively until the 7th day of inoculation. The minimum infective dose (MID) or the 50% infective dose (ID50) of the leptospiral suspension was determined by the appearance of the macroscopic local haemorrhage 7 days after inoculation. The MID thus determined was almost comparable with the value determined by the development of general symptoms and signs by conventional ip inoculation. The number of the pathogen per ID50 varied between 6 and 35 in five experiments. The local haemorrhage was effectively protected by active or passive immunization. Microscopically, haemorrhage at the inoculated site was found mainly in the dermis, directly beneath the epidermis in particular, and accompanied with leakage of the pathogen. The pathogen was also detected abunduntly in the thickened epidermal layer covering the inoculated area as well as in the epithelial matrix of hair-follicle, probably due to the proliferation of the pathogen at the site.
用出血性黄疸钩端螺旋体芝浦菌株对豚鼠进行实验性钩端螺旋体病研究。当将病原体皮内接种到动物背部时，在全身出血出现之前，接种部位观察到局部出血。局部病变的严重程度在接种后第7天之前逐渐增加。通过接种7天后宏观局部出血的出现来确定钩端螺旋体悬液的最小感染剂量（MID）或50%感染剂量（ID50）。如此确定的MID几乎与通过传统腹腔接种后全身症状和体征的发展所确定的值相当。在五个实验中，每ID50的病原体数量在6到35之间变化。主动或被动免疫可有效保护局部出血。在显微镜下，接种部位的出血主要发生在真皮层，特别是直接在表皮下方，并伴有病原体的渗漏。在覆盖接种区域的增厚表皮层以及毛囊的上皮基质中也大量检测到病原体，这可能是由于病原体在该部位增殖所致。
