Akama K, Otani S, Mori M, Arimitsu Y
Jpn J Med Sci Biol. 1975 Feb;28(1):1-9. doi: 10.7883/yoken1952.28.1.
A method for estimating the leptospiricidal activity of therapeutic antiserum for Weil's disease was improved by using the intracutaneous method in guinea pigs. The neutralization curves between the leptospiral suspensions for challenge and the antisera were shown to be linear over a wide range of the dosis of the pathogen. Although the reproducibility of the neutralization experiments was high, a significant divergence was observed among the slopes in various test samples. However, the trouble due to such a discrepancy in the slope may practically be reduced if such an antiserum preparation that has a regression coefficient close to the mean of those of various neutralization lines constructed by many different products is adopted as the reference. A practical method for the potency test was suggested.
通过在豚鼠身上采用皮内注射法，改进了一种用于估计韦尔氏病治疗抗血清杀钩端螺旋体活性的方法。在病原体剂量的很宽范围内，用于攻击的钩端螺旋体悬液与抗血清之间的中和曲线呈线性。虽然中和实验的可重复性很高，但在各种测试样品的斜率之间观察到了显著差异。然而，如果采用回归系数接近许多不同产品构建的各种中和线平均值的抗体制剂作为参考，实际上可以减少由于斜率差异引起的问题。提出了一种效价测试的实用方法。
