Latner A L, Turner G A, Cornell C
Exp Cell Biol. 1979;47(2):145-54. doi: 10.1159/000162931.
The effect of treatment with either whole calf thymus histones, or individually isolated histones, or polyarginine, or polylysine, on the nuclear size of BHK21 cells has been investigated. Incubation of the cells with mixed histones (12.5--44 microgram/ml) for 1 h considerably increased nuclear size. Increasing the histone concentration and/or the incubation time resulted in a decrease in the effect and could result in no change in nuclear size. Treatment of the cells with polyarginine or polylysine did not affect nuclear size. Experiments with individually isolated histones showed that the nuclear size effect was almost exclusively due to the histone H4. It is argued that the changes observed most likely resulted from interaction of H4 with the nucleus, and could reflect the properties of this particular histone molecule.
已经研究了用全小牛胸腺组蛋白、单独分离的组蛋白、聚精氨酸或聚赖氨酸处理对BHK21细胞细胞核大小的影响。将细胞与混合组蛋白（12.5 - 44微克/毫升）孵育1小时，可显著增加细胞核大小。增加组蛋白浓度和/或孵育时间会导致效果降低，甚至可能使细胞核大小没有变化。用聚精氨酸或聚赖氨酸处理细胞不会影响细胞核大小。对单独分离的组蛋白进行的实验表明，细胞核大小效应几乎完全归因于组蛋白H4。有人认为，观察到的变化很可能是由于H4与细胞核的相互作用导致的，并且可能反映了这种特定组蛋白分子的特性。
