染色质中组蛋白对DNA可及性的阻断：组蛋白的添加
Blocking by histones of accessibility to DNA in chromatin: addition of histones.
作者信息
Mirsky A E, Silverman B, Panda N C
出版信息
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3243-6. doi: 10.1073/pnas.69.11.3243.
Abstract
The blocking effect of histones on accessibility of DNA to DNase has been studied by addition of various histones to chromatin in intact or histone-depleted thymus nuclei (those from which all lysine-rich histone was removed and those from which much of the arginine-rich histone was removed) and to deoxyribonucleoprotein (chromatin) extracted from nuclei. In each case lysine-rich histone, weight for weight, blocks accessibility more effectively than does arginine-rich histone. Fractions of the arginine-rich histone differ considerably from each other in their blocking efficacy. Phosphorylation of lysine-rich histone tempers its blocking effectiveness.
摘要
通过向完整的或组蛋白缺失的胸腺细胞核（去除了所有富含赖氨酸的组蛋白的细胞核以及去除了大部分富含精氨酸的组蛋白的细胞核）中的染色质以及从细胞核中提取的脱氧核糖核蛋白（染色质）添加各种组蛋白，研究了组蛋白对DNA对脱氧核糖核酸酶可及性的阻断作用。在每种情况下，按重量计算，富含赖氨酸的组蛋白比富含精氨酸的组蛋白更有效地阻断可及性。富含精氨酸的组蛋白的不同组分在其阻断效力上有很大差异。富含赖氨酸的组蛋白的磷酸化会减弱其阻断效果。
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