大鼠肝脏中响应氢化可的松合成的RNA的性质

Nature of RNAs synthesised in rat liver in response to hydrocortisone.

作者信息

Kulkarni S B, Netrawali M S, Pradhan D S

出版信息

Mol Cell Endocrinol. 1979 Mar;13(3):269-80. doi: 10.1016/0303-7207(79)90086-8.

Abstract

Experiments on hybridization of [32P]HnRNA from hydrocortisone-treated rat liver with rat-liver DNA, in competition with unlabelled liver HnRNA from hormone-deficient and treated rats, revealed marked differences in their nucleotide sequence homologies. It was found that the hormone-induced new species of HnRNAs were transcribed both on middle-repetitive and non-repetitive sequences of DNA. However similar differences in the sequence homologies were not detectable in respect of mRNA derived from livers of hydrocortisone-administered and adrenalectomised rats. The results imply that the new species of HnRNA synthesized following hormone treatment may be confined to the nucleus.

摘要

用经氢化可的松处理的大鼠肝脏的[32P]HnRNA与大鼠肝脏DNA进行杂交实验，并与来自激素缺乏和经处理大鼠的未标记肝脏HnRNA竞争，结果显示它们的核苷酸序列同源性存在显著差异。研究发现，激素诱导产生的新HnRNA种类是在DNA的中度重复序列和非重复序列上转录的。然而，在来自给予氢化可的松和肾上腺切除大鼠肝脏的mRNA方面，未检测到类似的序列同源性差异。结果表明，激素处理后合成的新HnRNA种类可能局限于细胞核内。