大鼠肝脏再生过程中新的聚腺苷酸化核RNA序列的出现。
The appearance of new polyadenylated nuclear RNA sequences during rat-liver regeneration.
作者信息
Wilkes P R, Birnie G D
出版信息
Nucleic Acids Res. 1981 Apr 24;9(8):2021-35. doi: 10.1093/nar/9.8.2021.
Polyadenylated RNA populations from normal and 16-hour regenerating rat-liver nuclei were compared by heterologous hybridisation reactions with cDNA and unique DNA probes. Whereas unique DNA hybridisations did not show differences between the RNA populations, comparisons by cDNA hybridisation showed that about 10--15% by weight of polyadenylated sequences present in the nuclei of 16-hour regenerating rat livers were not found in the polyadenylated nuclear RNA of normal rat livers. These regenerating-specific nuclear cDNA sequences were isolated and characterised; the experiments showed that the complexity of the new sequences was 1-2 x 10(7) nucleotides (equivalent to 5,000--10,000 RNA sequences of 2,000 nucleotides in length) and that they were probably not potential messenger RNA sequences.
通过与互补DNA（cDNA）和单拷贝DNA探针的异源杂交反应，对正常大鼠肝脏细胞核和再生16小时的大鼠肝脏细胞核中的多聚腺苷酸化RNA群体进行了比较。单拷贝DNA杂交未显示RNA群体之间存在差异，但cDNA杂交比较表明，在再生16小时的大鼠肝脏细胞核中存在的多聚腺苷酸化序列中，约10%-15%（按重量计）在正常大鼠肝脏的多聚腺苷酸化核RNA中未发现。这些再生特异性核cDNA序列被分离并进行了表征；实验表明，新序列的复杂度为1-2×10⁷个核苷酸（相当于5000-10000个长度为2000个核苷酸的RNA序列），并且它们可能不是潜在的信使RNA序列。
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