Kostomarova A A, Nechaeva N V, Burakova T A
Ontogenez. 1974;5(5):463-9.
The concentration of dry substance (protein) and the dry weight of nuclei, cytoplasm and cells from different blastoderm regions at the early blastula and midgastrula stages were determined by interferentional microscopy. It was shown that at the early blastula stage the dry weight of cells in the basal layer is higher than that in the outer layer. Although the protein concentration in the basal layer cells appears to be somewhat higher, differences in their dry weight are due primarily to the big volume of cytoplasm of the basal layer cells. By the midgastrula stage, the total (nucleus + cytoplasm) protein concentration increases (by 17% in the basal layer cells and by 9% in the outer layer cells) due to the increase of nuclear protein concentration. At the same time dry weight of these cells markedly decreases due to the decrease of their volumes in the process of cell divisions. At the midgastrula stage the epiblast cells have the highest dry weight due to the highest protein concentration in the cytoplasm and the biggest cell volume. The results obtained are discussed with respect to the data on the pattern of accumulation of newly synthesized protein in nuclei and cytoplasm with special reference to the duration of individual cell cycle phases.
采用干涉显微镜测定了囊胚早期和原肠胚中期不同胚盘区域细胞核、细胞质及细胞的干物质（蛋白质）浓度和干重。结果表明，在囊胚早期，基底层细胞的干重高于外层细胞。虽然基底层细胞中的蛋白质浓度似乎略高，但其干重差异主要是由于基底层细胞的细胞质体积较大。到原肠胚中期，由于核蛋白浓度增加，总（细胞核+细胞质）蛋白质浓度升高（基底层细胞增加17%，外层细胞增加9%）。与此同时，由于细胞分裂过程中细胞体积减小，这些细胞的干重显著降低。在原肠胚中期，外胚层细胞由于细胞质中蛋白质浓度最高且细胞体积最大，其干重最高。结合细胞核和细胞质中新合成蛋白质积累模式的数据，特别是关于各个细胞周期阶段的持续时间，对所得结果进行了讨论。
