Felby S
Forensic Sci Int. 1979 Mar-Apr;13(2):145-50. doi: 10.1016/0379-0738(79)90278-0.
A method for the determination of nanogram quantities of morphine in hemolyzed whole blood taken post mortem, or other blood samples of poor quality, has been developed. Morphine was extracted into ethyl acetate from blood at pH 8.7-9.0, and purified by absorption onto a column of acid aluminium oxide and elution with methanol. Nalorphine was added as an internal standard, the compounds derivatized with pentafluoropropionic acid anhydride, and the derivatives formed quantified by electron-capture gas chromatography. The recovery of morphine added to whole blood was 83%, the coefficient of variation 6.7%. The method is capable of measuring morphine concentrations of 1 ng/g in 0.1-0.2 g of post-mortem blood.
已开发出一种用于测定死后采集的溶血全血或其他质量较差的血液样本中纳克量吗啡的方法。在pH 8.7 - 9.0条件下，将吗啡从血液中萃取到乙酸乙酯中，通过吸附到酸性氧化铝柱上并用甲醇洗脱进行纯化。加入烯丙吗啡作为内标，用五氟丙酸酐对化合物进行衍生化，通过电子捕获气相色谱法对形成的衍生物进行定量。添加到全血中的吗啡回收率为83%，变异系数为6.7%。该方法能够测定0.1 - 0.2 g死后血液中1 ng/g的吗啡浓度。
