英国亲子鉴定案例中通过等电聚焦和淀粉凝胶电泳对红细胞磷酸葡萄糖变位酶1（PGM1）进行表型分析。对95个案例所获结果的评估。

Red cell PGM1 (phosphoglucomutase) phenotyping by isoelectric focussing and starch gel electrophoresis in cases of disputed paternity in the United Kingdom. An evaluation of the results obtained in 95 cases.

作者信息

Welch S G, Wood N J, Dodd B E

出版信息

Forensic Sci Int. 1979 Mar-Apr;13(2):87-92. doi: 10.1016/0379-0738(79)90268-8.

Abstract

Using two techniques for red cell PGM1 (phosphoglucomutase) phenotyping, namely starch gel electrophoresis and isoelectric focussing, a parallel study has been carried out in 95 paternity cases. The results again confirm the genetic basis for the extended polymorphism revealed by isoelectric focussing. Fifteen men were excluded from paternity on the basis of isoelectric focussing compared with seven excluded by starch gel electrophoresis. This was found to be in good agreement with the predicted increase in exclusion rate that the isoelectric focussing technique for red cell PGM1 provides.

摘要

采用淀粉凝胶电泳和等电聚焦这两种红细胞磷酸葡萄糖变位酶1（PGM1）表型分析技术，对95例亲子鉴定案例进行了平行研究。结果再次证实了等电聚焦所揭示的扩展多态性的遗传基础。基于等电聚焦，有15名男性被排除亲子关系，而基于淀粉凝胶电泳则有7名被排除。结果发现这与红细胞PGM1等电聚焦技术所提供的预期排除率增加情况高度吻合。