Isoelectric focusing of PGM1 (E.C.2.7.5.1) on agarose. Application to cases of disputed parentage.

A method for isoelectric focusing of phosphoglucomutase (PGM1) using agarose was found to have significant advantages over published acrylamide technics. Gene frequencies determined on samples from 1,780 Minnesota Caucasians were PGM1(1+) = 0.6118, PGM1(1-) = 0.1441, PGM1(2+) = 0.1910, PGM1(2-) = 0.0531. The use of the isoelectric focusing technics in 700 cases of disputed parentage resulted in an exclusion rate slightly in excess of the calculated probability of exclusion (P value) of 27%.. In addition, the proportions of direct exclusions was increased from 485 observed with the conventional starch technic to 75% with IEF.