赫氏疏螺旋体在凯利培养基中的生物学特性
Biology of Borrelia hermsii in Kelly medium.
作者信息
Stoenner H G
出版信息
Appl Microbiol. 1974 Oct;28(4):540-3. doi: 10.1128/am.28.4.540-543.1974.
Abstract
More than 800 Borellia hermsii in mouse plasma were required for establishment of growth in an artificial medium (Kelly), but only a single organism of a fully adapted strain (25th subculture) was required for a successful subculture. As judged by generation time, maximal concentration in culture, and length and motility of the organism, the process of adaptation extended through at least 11 subcultures. Because the organisms regularly died shortly after the logarithmic growth phase, transfers at 7- to 10-day intervals were required to maintain continuous cultures.
摘要
在人工培养基（凯利培养基）中培养时，小鼠血浆中需要800多个赫氏疏螺旋体才能开始生长，但对于一个完全适应的菌株（第25代传代培养物），成功传代仅需要一个生物体。从代时、培养物中的最大浓度以及生物体的长度和运动性判断，适应过程至少持续了11代传代培养。由于这些生物体在对数生长期后不久就经常死亡，因此需要每隔7至10天进行传代培养以维持连续培养。
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