Barbour A G, Todd W J, Stoenner H G
Antimicrob Agents Chemother. 1982 May;21(5):823-9. doi: 10.1128/AAC.21.5.823.
Borrelia hermsii, a spirochete and an etiological agent of relapsing fever, was cultivated in modified Kelly medium. Studies of the action of penicillin on B. hermsii strain HS1 revealed the following: (i) the in vitro minimum inhibitory concentration and minimum bactericidal concentration of benzylpenicillin for this strain were 0.4 and 3.1 nmol/ml (0.15 and 1.1 micrograms/ml), respectively; (ii) the primary morphological responses at the minimum bactericidal concentration of benzylpenicillin were the formation of spheroplast-like structures and an increased number of small, membranous blebs; (iii) radioactive benzylpenicillin bound to five penicillin-binding proteins in the whole cells of B. hermsii. The 50% binding concentrations of labeled penicillin for the five penicillin-binding proteins were within a factor of five of the minimum inhibitory concentration. More than one-half of the total bound labeled penicillin was associated with penicillin-binding protein 1, the penicillin-binding protein with the largest apparent molecular weight (90,000).
赫氏疏螺旋体是一种螺旋体，也是回归热的病原体，它在改良的凯利培养基中培养。对青霉素作用于赫氏疏螺旋体HS1菌株的研究结果如下：（i）该菌株对苄青霉素的体外最低抑菌浓度和最低杀菌浓度分别为0.4和3.1 nmol/ml（0.15和1.1微克/毫升）；（ii）在苄青霉素最低杀菌浓度下的主要形态学反应是形成类原生质体结构和数量增加的小膜泡；（iii）放射性苄青霉素与赫氏疏螺旋体全细胞中的五种青霉素结合蛋白结合。标记青霉素对这五种青霉素结合蛋白的50%结合浓度在最低抑菌浓度的五倍范围内。超过一半的总结合标记青霉素与青霉素结合蛋白1相关，该青霉素结合蛋白具有最大的表观分子量（90,000）。
