Moore T, Sharman I M
Int J Vitam Nutr Res. 1979;49(1):14-20.
Weanling male rats were fed for 24 days on a meat diet. Some received supplements of CaCO3, but others did not. For the final 14 days of the experiment some of the rats were given massive doses of retinol as retinyl acetate, but others were undosed. Some of the rats not given retinol had their food intakes paired with those given retinol. Skeletal examinations were made by radiography, and postmortem by measurement of the femur ash contents. The meat diet regularly reduced the femur ash to about half its normal level, but the CaCO3 supplements prevented any reduction. Skeletal fractures occurred consistently in all the rats given retinol, and with little if any difference in severity between the Ca-deficient and Ca-supplemented animals. Histological studies confirmed the accumulation of abnormal amounts of lipid round the liver Kupffer cells in hypervitaminosis A. Chemical estimations of liver fat gave higher values for the retinol-dosed animals than for their controls on paired food intakes. For control rats on unrestricted food intakes, however, higher values than for the retinol-dosed animals were found, but without lipid depositions round the Kupffer cells.
将断乳雄性大鼠用肉类饲料喂养24天。一些大鼠补充碳酸钙，而另一些则不补充。在实验的最后14天，给一些大鼠大剂量的视黄醇醋酸酯，但另一些大鼠不给予。一些未给予视黄醇的大鼠的食物摄入量与给予视黄醇的大鼠配对。通过X射线摄影进行骨骼检查，并在死后测量股骨灰分含量。肉类饲料通常会使股骨灰分降至正常水平的约一半，但补充碳酸钙可防止任何降低。所有给予视黄醇的大鼠均持续发生骨骼骨折，缺钙和补钙动物之间的严重程度差异很小或没有差异。组织学研究证实，维生素A过多症时肝脏库普弗细胞周围异常脂质积聚。对肝脏脂肪的化学测定显示，给予视黄醇的动物的肝脏脂肪值高于配对食物摄入量的对照组。然而，对于食物摄入量不受限制的对照大鼠，发现其肝脏脂肪值高于给予视黄醇的动物，但库普弗细胞周围没有脂质沉积。
