用软骨素酶从硫酸软骨素产生的不饱和二糖的高效液相色谱法
High performance liquid chromatography of unsaturated disaccharides produced from chondroitin sulfates by chondroitinase.
作者信息
Ototani N, Sato N, Yosizawa Z
出版信息
J Biochem. 1979 May;85(5):1383-5.
PMID:447623
Abstract
High performance liquid chromatography was performed by the ion pair method on unsaturated disaccharides produced from chondroitin sulfates by the action of chondroitinase. Completely separated peaks corresponding to deltaDi-0S, deltaDi-4S, and deltaDi-6S were obtained on a column of mu-bondapak-C18 with 0.035 M tetra-butylammonium phosphate (pH 7.54) as a mobile phase. There was a linear relationship between the ratio of the peak area and the amount of each standard tested.
摘要
采用离子对法，通过高效液相色谱对硫酸软骨素经软骨素酶作用产生的不饱和二糖进行分析。以0.035M磷酸四丁铵（pH 7.54）为流动相，在μ-邦德帕克-C18柱上得到了对应于ΔDi-0S、ΔDi-4S和ΔDi-6S的完全分离的峰。峰面积比与各测试标准品的量之间存在线性关系。
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