小牛胸腺DNA在组蛋白KAP-琼脂糖凝胶4B柱上的分级分离。
The fractionation of calf thymus DNA on histone KAP-Sepharose 4B columns.
作者信息
Panusz H, Bartkowiak J, Krzywiec A, Joustra M, Plucienniczak A
出版信息
Nucleic Acids Res. 1974 Sep;1(9):1143-51. doi: 10.1093/nar/1.9.1143.
It was found that fractionation of calf thymus DNA on homologous histtone KAP covalently bound to CNBr activated Sepharose 4B depends on the molecular weight of DNA. The maximum of elution of high molecular DNA (m. wt. above 5 x 10(6)) was observed at 0.56 M NaCl and that of degraded DNA (m. wt. 0.8 x 10(6)) at 0.52 M NaCl. Significant differences in melting temperatures and melting intervals were observed among fractions obtained from low molecular DNA as a result of enrichment of some fractions in satellite DNAs. These differences were very small in DNA of m. wt. above 5 x 10(6). The results are discussed in terms of specific areas which may exist on calf thymus DNA molecules playing the role of loci, where lysine-rich histone KAP is preferentially bound.
发现将小牛胸腺DNA在共价结合于溴化氰活化的琼脂糖凝胶4B的同源组蛋白KAP上进行分级分离取决于DNA的分子量。高分子量DNA（分子量高于5×10⁶）在0.56M氯化钠时洗脱量最大，而降解DNA（分子量0.8×10⁶）在0.52M氯化钠时洗脱量最大。由于某些级分中卫星DNA的富集，从低分子量DNA获得的级分之间观察到熔解温度和熔解区间存在显著差异。在分子量高于5×10⁶的DNA中这些差异非常小。根据小牛胸腺DNA分子上可能存在的特定区域讨论了这些结果，这些区域起基因座的作用，富含赖氨酸的组蛋白KAP优先结合于此。
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