通过与同源富含赖氨酸的组蛋白KAP多次形成可沉降复合物对小牛胸腺DNA进行分级分离。衍生熔解曲线和在CsCl浓度梯度中的超速离心。
The fractionation of calf thymus DNA by multiple formation of sedimentable complexes with homologous lysine-rich histone KAP. Derivative melting curves and ultracentrifugation in CsCl concentration gradients.
作者信息
Plucienniczak A, Bartkowiak J, Krzywiec A, Panusz H
出版信息
Nucleic Acids Res. 1974 Dec;1(12):1675-90. doi: 10.1093/nar/1.12.1675.
Abstract
The process of fractionation of total calf thymus DNA using a step precipitation of DNA by means of increasing concentrations of the homologous histone KAP was investigated. In addition to the known fractions three so far undescribed ones/in thymus/,characterized by buoyant densities in CsCl equal 1.692, 1.706 and 1.728 g/ccm, were identified. Considerable amounts of preparations seriously enriched in individual satellite fractions were obtained. The ability of GC-rich satellite DNAs to form more soluble complexes with histone KAP is suggested as reason for the observed fractionation.
摘要
研究了利用同源组蛋白KAP浓度递增对小牛胸腺总DNA进行分步沉淀的分级分离过程。除了已知的组分外，还鉴定出了迄今未描述的三种组分（在胸腺中），其在CsCl中的浮力密度分别为1.692、1.706和1.728 g/ccm。获得了大量严重富集于各个卫星组分的制剂。富含GC的卫星DNA与组蛋白KAP形成更易溶复合物的能力被认为是观察到的分级分离的原因。
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