Woodland H, Ayers S E
Biochem J. 1974 Oct;144(1):11-9. doi: 10.1042/bj1440011.
Micro-injection into the oocytes and eggs of Xenopus laevis was used to ascertain the effects of synthetic polyribonucleotides on protein synthesis in living cells. Poly(U) and poly(A) were not translated detectably, nor did they change the rate of endogenous protein synthesis. The same was true of poly(G,U), poly(A,G,U), poly(A,C,G,U), G-U-G-(U)(n), A-(U)(n) and AUG. In contrast, A-U-G-(U)(n) was a potent inhibitor of protein synthesis in the cell. This might be because it is initiated normally but lacks a termination codon, or because it inhibits the translation of other molecules in some way not dependent on its normal initiation. Poly(G,U), poly(A,G,U) and poly(A,C,G,U) inhibited haemoglobin synthesis when they were injected into the oocyte with haemoglobin mRNA. The synthetic polyribonucleotides did not inhibit the translation of the natural mRNA when the two sorts of molecules were injected at different times. It is suggested that the synthetic RNA molecules compete with the natural mRNA for a pre-initiation factor in limited supply.
通过向非洲爪蟾的卵母细胞和卵中进行显微注射，来确定合成多聚核糖核苷酸对活细胞中蛋白质合成的影响。聚尿苷酸（Poly(U)）和聚腺苷酸（Poly(A)）无法被检测到翻译，它们也不会改变内源性蛋白质合成的速率。聚鸟苷酸尿苷酸（Poly(G,U)）、聚腺苷酸鸟苷酸尿苷酸（Poly(A,G,U)）、聚腺苷酸胞苷酸鸟苷酸尿苷酸（Poly(A,C,G,U)）、G-U-G-(U)(n)、A-(U)(n)和AUG也是如此。相比之下，A-U-G-(U)(n)是细胞中蛋白质合成的有效抑制剂。这可能是因为它正常起始但缺乏终止密码子，或者是因为它以某种不依赖于其正常起始的方式抑制其他分子的翻译。当聚鸟苷酸尿苷酸（Poly(G,U)）、聚腺苷酸鸟苷酸尿苷酸（Poly(A,G,U)）和聚腺苷酸胞苷酸鸟苷酸尿苷酸（Poly(A,C,G,U)）与血红蛋白mRNA一起注射到卵母细胞中时，它们会抑制血红蛋白的合成。当两种分子在不同时间注射时，合成多聚核糖核苷酸不会抑制天然mRNA的翻译。有人提出，合成RNA分子与天然mRNA竞争有限供应的起始前因子。
