Bienz M, Kubli E, Kohli J, deHenau S, Huez G, Marbaix G, Grosjean H
Nucleic Acids Res. 1981 Aug 11;9(15):3835-50. doi: 10.1093/nar/9.15.3835.
Oocytes from Xenopus laevis were injected with purified amber (UAG), ochre (UAA), and opal (UGA) suppressor tRNAs from yeasts. The radioactively labeled proteins translated from the endogenous mRNAs were then separated on two-dimensional gels. All three termination codons are used in a single cell, the Xenopus laevis oocyte. But a surprisingly low number of readthrough polypeptides were observed from the 600 mRNAs studied in comparison to uninjected oocytes. The experimental data are compared with the conclusions obtained from the compilation of all available termination sequences on eukaryotic and prokaryotic mRNAs. This comparison indicates that the apparent resistance of natural termination codons against readthrough, as observed by the microinjection experiments, cannot be explained by tandem or very close second stop codons. Instead it suggests that specific context sequences around the termination codons may play a role in the efficiency of translation termination.
将来自酵母的纯化琥珀色（UAG）、赭石色（UAA）和乳白（UGA）抑制性tRNA注射到非洲爪蟾的卵母细胞中。然后，从内源性mRNA翻译得到的放射性标记蛋白质在二维凝胶上进行分离。在单个细胞即非洲爪蟾卵母细胞中，所有三个终止密码子都被使用。但是，与未注射的卵母细胞相比，在所研究的600种mRNA中观察到的通读多肽数量出奇地低。将实验数据与从真核和原核mRNA上所有可用终止序列汇编中得出的结论进行了比较。这种比较表明，显微注射实验中观察到的天然终止密码子对通读的明显抗性，不能用串联或非常接近的第二个终止密码子来解释。相反，这表明终止密码子周围的特定上下文序列可能在翻译终止效率中起作用。
