Kuttner B J, Woodruff J J
J Immunol. 1979 May;122(5):1666-71.
An in vitro system developed for studying lymphocyte binding to high endothelial venules (HEV) of lymph nodes was used to determine if there are similar binding sites in other organs of the rat. Thoracic duct lymphocytes (TDL) adhered selectively and uniformly to white matter when overlaid onto glutaraldehyde-fixed tissue sections of cerebellum and cerebrum. The pattern of TDL adherence to cerebellar sections showed that binding to nonmyelinated areas was negligible. Comparison of TDL-white matter to TDL-HEV binding demonstrated that the density of adherence to each site was quantitatively similar. In contrast, lymphocytes exhibited little tendency to bind to tissue sections of liver, spleen, heart, thymus, and salivary glands. TDL adherence to cerebellar white matter occurred rapidly, was cell dose dependent and optimal at 7 degrees C. White matter binding was also a property of spleen lymphocytes but the thymus was deficient in cells with this capability. The affinity of TDL and spleen lymphocytes for myelinated areas of the brain suggests the presence of myelin binding receptors on these cells.
一种用于研究淋巴细胞与淋巴结高内皮微静脉（HEV）结合的体外系统，被用来确定大鼠的其他器官中是否存在类似的结合位点。当将胸导管淋巴细胞（TDL）覆盖在小脑和大脑的戊二醛固定组织切片上时，它们选择性且均匀地粘附于白质。TDL对小脑切片的粘附模式表明，其与无髓鞘区域的结合可忽略不计。将TDL与白质的结合和TDL与HEV的结合进行比较，结果表明，每个位点的粘附密度在数量上相似。相比之下，淋巴细胞与肝脏、脾脏、心脏、胸腺和唾液腺的组织切片几乎没有结合倾向。TDL对小脑白质的粘附迅速，呈细胞剂量依赖性，在7摄氏度时最佳。白质结合也是脾淋巴细胞的特性，但胸腺缺乏具有这种能力的细胞。TDL和脾淋巴细胞对大脑有髓鞘区域的亲和力表明，这些细胞上存在髓鞘结合受体。
