de Rezende T A, Ribeiro M A, Campana A O
Arq Gastroenterol. 1979 Jul-Sep;16(3):114-8.
The experiment was performed in order to evaluate the beta-glucuronidase activity in gastric juice and gastric mucosa of rats submitted to protein-free diet. A group of 36 young adult male wistar rats was fed a protein-free diet ad libitum for five weeks; a second group of 36 wistar rats ingested a purified isocaloric 12,5% casein diet for the same period. The concentration of proteins in plasma, gastric juice and gastric glandular mucosa and the beta-glucuronidase activity in the gastric juice and gastric glandular mucosa were determined. Protein deficient rats had lower plasma protein concentration and also a lower protein concentration in gastric juice and gastric mucosa. In these animals there was no significant change of beta-glucuronidase activity in the gastric juice, but there was a significant increase of the specific enzimatic activity in the gastric mucosa. The results suggest that protein restriction in young adult rats affects the gastric mucosa. The increase of the specific beta-glucuronidase activity might be due to heightened local catabolism or to a comparatively more severe protein depletion.
进行该实验是为了评估无蛋白饮食大鼠胃液和胃黏膜中的β-葡萄糖醛酸酶活性。一组36只成年雄性Wistar大鼠自由进食无蛋白饮食5周；另一组36只Wistar大鼠在同一时期摄入纯化的等热量12.5%酪蛋白饮食。测定了血浆、胃液和胃腺黏膜中的蛋白质浓度以及胃液和胃腺黏膜中的β-葡萄糖醛酸酶活性。蛋白质缺乏的大鼠血浆蛋白浓度较低，胃液和胃黏膜中的蛋白质浓度也较低。在这些动物中，胃液中β-葡萄糖醛酸酶活性没有显著变化，但胃黏膜中的比酶活性显著增加。结果表明，成年幼鼠蛋白质限制会影响胃黏膜。比β-葡萄糖醛酸酶活性的增加可能是由于局部分解代谢增强或蛋白质消耗相对更严重。
