Irwin D, Malt R A
Lab Invest. 1979 Jul;41(1):77-82.
A possible role of messenger RNA (mRNA)-associated proteins during compensatory renal hypertrophy in modulation of mRNA biogenesis has been examined in this investigation. Renal cytoplasmic free messenger ribonucleoprotein (CF mRNP) and polysomal mRNP (PA mRNP) have been isolated from kidney following 48 hours of compensatory renal hypertrophy, and the relative amounts of proteins associated with CF mRNP and PA mRNP in control and hypertrophying mouse kidney are compared. (CF mRNP refers to nonpolysomal messenger ribonucleoprotein found in the subribosomal region of sucrose gradients. PA mRNP or polysome-associated mRNP refers to mRNP released from polysomes.) CF mRNP has a buoyant density of 1.3 to 1.44 gm. per ml., contains RNA that sediments at 5 to 22 Svedberg units, and has a protein moiety comprising at least 15 polypeptides as revealed by sodium dodecyl sulfate polyacrylamide gels. PA mRNP contains three major polypeptides (molecular weights, 77,000, 52,000 and 17,000). Following 48 hours of compensatory renal hypertrophy, the relative amounts of proteins are changed: a 44,000- and 52,000- molecular weight polypeptide in CF mRNP is increased, a 52,000-molecular weight polypeptide in PA mRNP is reduced, and a 17,000-molecular weight polypeptide in PA mRNP is increased.
本研究探讨了信使核糖核酸（mRNA）相关蛋白在代偿性肾肥大过程中对mRNA生物合成的调节作用。在代偿性肾肥大48小时后，从肾脏中分离出肾细胞质游离信使核糖核蛋白（CF mRNP）和多聚核糖体mRNP（PA mRNP），并比较了对照小鼠和肥大小鼠肾脏中与CF mRNP和PA mRNP相关的蛋白质相对含量。（CF mRNP是指在蔗糖梯度的亚核糖体区域发现的非多聚核糖体信使核糖核蛋白。PA mRNP或多聚核糖体相关mRNP是指从多聚核糖体释放的mRNP。）CF mRNP的浮力密度为每毫升1.3至1.44克，含有沉降系数为5至22 Svedberg单位的RNA，并且如十二烷基硫酸钠聚丙烯酰胺凝胶所示，其蛋白质部分包含至少15种多肽。PA mRNP包含三种主要多肽（分子量分别为77,000、52,000和17,000）。在代偿性肾肥大48小时后，蛋白质的相对含量发生了变化：CF mRNP中分子量为44,000和52,000的多肽增加，PA mRNP中分子量为52,000的多肽减少，PA mRNP中分子量为17,000的多肽增加。
