[小鼠浆细胞瘤细胞胞质中含信使核糖核酸的核糖核蛋白]

[Ribonucleoproteins containing mRNA in the cytoplasm of mouse plasmacytoma cells].

作者信息

Gortiunova L E, Sakharova N K, Grechko V V

出版信息

Mol Biol (Mosk). 1975 Nov-Dec;9(6):922-33.

Abstract

The rapidly labelled postribosomal ribonucleoprotein (RNP) found in the cytoplasm of mouse plasmacytoma cells were investigated. It has been shown that 45S and 80S particles contain relatively high molecular weight (approximately 12-17S) pulse-labelled RNA similar to the polyribosomal mRNA. No other postribosomal RNP was found which would contain an RNA with similar sedimentation characteristics. In CsC1 density gradients, the postribosomal RNP gives two peaks. One of them, the rapidly labelled component (rho 1.52 g/cm3) is found only in 45S RNP. The other rapidly labelled component (rho 1.36-1.41 g/cm3) is revealed in all investigated regions of sucrose gradients. The latter contains relatively low molecular weight RNA (approximately7-9S). These RNP are supposed to be informosome-like particles. The components with a buoyant density of 1.52 g/cm3 may represent an mRNP-45S subparticles complex. The rapidly labelled mRNA of 80S particles is released after EDTA treatment in the form of mRNP with a buoyant density of 1.45-1.47 g/cm3.

摘要

对在小鼠浆细胞瘤细胞质中发现的快速标记的核糖体后核糖核蛋白（RNP）进行了研究。结果表明，45S和80S颗粒含有相对高分子量（约12 - 17S）的脉冲标记RNA，类似于多核糖体mRNA。未发现其他核糖体后RNP含有具有相似沉降特性的RNA。在CsC1密度梯度中，核糖体后RNP给出两个峰。其中一个，快速标记成分（ρ1.52 g/cm³）仅在45S RNP中发现。另一个快速标记成分（ρ1.36 - 1.41 g/cm³）在蔗糖梯度的所有研究区域中都有显示。后者含有相对低分子量的RNA（约7 - 9S）。这些RNP被认为是类信息体颗粒。浮力密度为1.52 g/cm³的成分可能代表mRNP - 45S亚颗粒复合物。80S颗粒的快速标记mRNA在EDTA处理后以浮力密度为1.45 - 1.47 g/cm³的mRNP形式释放。